![]() There is no apparent genotoxic effect from exposure of sperm to Hoechst 33342, although information on cellular toxicity or development of embryos resulting from Hoechst 33342-stained sperm is less reassuring. Offspring with obvious abnormalities are no more frequent than after insemination of unsorted sperm into cows, horses, humans, pigs, sheep, rabbits, dolphins and other mammals. Capability to distinguish and separate X- and Y-chromosome-bearing sperm has led to artificial insemination of somewhere around a million female mammals. The molecule permeates the cell membrane of living cells and binds selectively to A-T base pairs exposed in the minor-groove of double stranded DNA. This synthetic dye consists of two adjacent benzimidazole rings with N-methyl-piperazine and phenolic groups at the ends. A difference of <3% in DNA mass can be detected. Hoechst 33342 is the fluorophore used routinely to measure DNA in X- and Y-chromosome-bearing mammalian sperm so they can be separated by flow sorting. Un algoritmo de aprendizaje automático puede usar parámetros clínicos y marcadores de capacitación para predecir con precisión la fecundación exitosa en hombres normospérmicos sometidos a FIV convencional. En el análisis de la curva de funcionamiento del receptor de los datos del equipo de prueba (n = 58), el algoritmo de aprendizaje automático predijo una IVF convencional exitosa con una precisión media de 0,72 (n = 18), un área media debajo de la curva de 0,81, una sensibilidad media de 0,65 y una especificidad media de 0,80.Įl pHi seminal se correlaciona con los resultados de la IVF convencional en pacientes normospérmicos que realizan IVF. Fueron excluidos los pacientes que utilizaban semen congelado, tenían factor masculino conocido o sólo realizaron inyección intracitoplasmática de espermatozoides.Įl pHi seminal se correlacionó positivamente con la motilidad hiperactivada y con la tasa de IVF convencional (n=76) pero no con la tasa de fertilización intracitoplasmática (n=58). Hombres normospérmicos que realizaron IVF. El algoritmo fue validado para un conjunto de datos independiente de 18 pacientes. Se entrenó un algoritmo de aprendizaje automático impulsado por gradientes basado en datos clínicos y pHi espermático y potencial de membrana de 58 pacientes para predecir la IVF convencional exitosa, definida como tasa de fecundación (número de ovocitos fecundados /número de ovocitos maduros) mayor de 0.66. Se utilizó la citometría de flujo para medir el pHi seminal, y se midió la motilidad hiperactivada mediante análisis seminal asistido por computadora. Se capacitaron in vitro los espermatozoides de 76 pacientes. Medir el pH intracelular del esperma humano (pHi) y desarrollar un algoritmo de aprendizaje automático para predecir el éxito de la fertilización in vitro (IVF) convencional en pacientes normospérmicos. A machine-learning algorithm can use clinical parameters and markers of capacitation to accurately predict successful fertilization in normospermic men undergoing conventional IVF.Įl algoritmo de aprendizaje automático incorporando el ph intracelular de espermatozoides capacitados predice el éxito de la fertilización in vitro convencional en pacientes normospérmicos. Sperm pH i correlates with conventional fertilization outcomes in normospermic patients undergoing IVF. In receiver operating curve analysis of data from the test set (n = 58), the machine-learning algorithm predicted successful conventional IVF with a mean accuracy of 0.72 (n = 18), a mean area under the curve of 0.81, a mean sensitivity of 0.65, and a mean specificity of 0.80. Sperm pH i positively correlated with hyperactivated motility and with conventional IVF ratio (n = 76) but not with intracytoplasmic sperm injection fertilization ratio (n = 38). Patients were excluded if they used frozen sperm, had known male factor infertility, or used intracytoplasmic sperm injection only. The algorithm was validated on an independent set of data from 18 patients. A gradient-boosted machine-learning algorithm was trained on clinical data and sperm pH i and membrane potential from 58 patients to predict successful conventional IVF, defined as a fertilization ratio (number of fertilized oocytes /number of mature oocytes) greater than 0.66. Flow cytometry was used to measure sperm pH i, and computer-assisted semen analysis was used to measure hyperactivated motility. Spermatozoa from 76 IVF patients were capacitated in vitro. To measure human sperm intracellular pH (pH i) and develop a machine-learning algorithm to predict successful conventional in vitro fertilization (IVF) in normospermic patients.
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